重慶市華雅干細胞技術有限公司 ，  ：

華雅集團旗下授權代理TTAKARA Cellartis干細胞產品

圖片為展示效果，實際產品為hES-MP 002.5細胞株

高礦化潛力的間充質前體細胞

Cellartis-Y10090 hES-MP 002.5人類胚胎干細胞來源的間充質前體細胞

產品英文名稱：hES-MP 002.5，Human Multipotent Mesenchymal Progenitor Cells

產品中文名稱：人類胚胎干細胞來源的間充質前體細胞

hES-MP 002.5是從人胚胎干細胞誘導分化而得到的間充質干細胞前體細胞（multipotent mesenchymal progenitors）。hES-MP 002.5與人間充質干細胞（human mesenchymal stem， MS）高度相似，可以分化成為多種中胚層細胞種類。hES-MP 002.5由人胚胎干細胞系SA002.5 ES（a subclone of SA002）分化得來，可以向骨細胞進行高效率的分化，分化過程具有著高級別的礦物化潛力（mineralization potential）。hES-MP 002.5細胞產品是高度均勻的、高潛力的間充質干細胞前體細胞，適合于科研級別以及工業規模的間充質干細胞生產。

hES-MP 002.5 cells are human embryonic stem (ES) cell-derived multipotent mesenchymal progenitors that resemble adult human mesenchymal stem (MS) cells and can be differentiated into several mesodermal lineages. The cells are derived from the SA002.5 ES cell line, a subclone of SA002, known for efficient osteogenic lineage differentiation and high mineralization potential with extended culture. hES-MP 002.5 cells are a homogeneous, robust source of mesenchymal progenitors, ideal for bulk production of mesenchymal cells.

產品特點

均質的，批次檢測的細胞群; 沒有供體到供體的可變問題

細胞強壯易培養

與成人人類間充質干細胞高度相似

能夠分化成中胚層譜系

擴增后高礦化潛力High mineralization potential with extended expansion

應用

間充質誘導和分化研究

修復和再生的組織工程模型

Mesenchymal induction and differentiation studies

Tissue engineering models of repair and regeneration

2017應用實例：

研究人員使用Cellartis® hES-MP 002.5細胞表明了流動體可以刺激間充質干細胞的骨基質產生。

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Sasima Puwanun  Robin M. Delaine‐Smith  Helen E. Colley  Julian M. Yates  Sheila MacNeil Gwendolen C. Reilly

Journal of Tissue Engineering and Regenerative Medicine,First published: 9 May 2017 //doi.org/10.1002/term.2462

組份

1瓶細胞

1 frozen vial of Cellartis® hES-MP 002.5

保存：≤ -150°C。

使用方法

Unpacking of Cellartis® hES-MP 002.5

NOTE! For your protection: Wear a protective face mask and protective gloves. Use forceps when

handling a frozen vial. Never hold the vial in your hand as the cryo vial may explode due to rapid

temperature changes.

NOTE! When transferring the cells from the transport vessel to long time storage, immediate transfer is essential since variations in temperature may have an adverse effect on cell survival and quality.

1. Unpack Cellartis® hES-MP 002.5 as soon as possible after arrival.

2. Check all materials for leakage or breakage.

3. Using forceps immediay place the vial in liquid nitrogen at ≤ -150°C.

自備試劑：

產品                                     建議的制造商            貨號

basic Fibroblast Growth Factor (bFGF)            Peprotech              100-18B

Dulbecco’s Modified Eagle Medium (DMEM)    Gibco / Life Technologies   31966-021

胎牛血清（FBS）                          Gibco / Life Technologies   16140

明膠                                     Sigma-Aldrich            G1890

PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS (-/-)) Gibco / Life Technologies    14190

青霉素/鏈霉素（PEST）                    Gibco / Life Technologies    15140-122

TrypLE Select™                            Gibco / Life Technologies    12563-011

產品性能

Cellartis hES-MP 002.5細胞傳30代，細胞群倍加數（cell population doubling level，縮寫PDL）沒有下降。

Population doubling of human mesenchymal stem cells (hMSCs) and Cellartis hES-MP 002.5 cells through 30 passages. Unlike hMSCs, hES-MP 00.25 cells show no decrease in population doubling level.

訂購信息

?華雅集團旗下授權代理TTAKARA Cellartis干細胞產品，Cellartis的多能細胞培養系統包括iPS誘導多能干細胞株、iPS誘導多能干細胞和ES胚胎干細胞培養基，iPS基因編輯用單細胞克隆培養基和多能細胞檢測用單克隆抗體。

重慶市華雅干細胞技術有限公司以“專注干細胞研究，提升干細胞品質”為發展理念，專業從事干細胞基礎研究、干細胞再生醫學臨床應用研究及干細胞技術服務，自主研發產品熱賣中：人間充質干細胞無血清培養基 

咨詢Cellartis-Y10090 Human Multipotent Mesenchymal Progenitor cells ，  ：